The CCF is divided in two floors, each one comprising three working rooms, equipped with state-of-the-art equipment including CO₂ incubators and laminal flow hoods (in a total of seven and six, respectively), and a support room, equipped with a dedicated inverted microscope, a refrigerator/freezer, and a centrifuge. The rooms are divided according to specific usages:

• Four rooms for general use (with mammalian cultures). These rooms comprise two CO₂ incubators intended for human-derived established cell lines, one for human-derived primary cultures and biomaterials, one for cultures of other animal origins (including explants and organotypic cultures), and one for quarantine (for cultures from external sources) and other special needs (e.g., mycoplasma testing);
• One dedicated to stem cells-related cultures (e.g., nanospheres), comprising a dedicated CO₂ incubator; 
• One to virus manipulation (e.g., lentiviral-based transduction of cells), comprising a dedicated CO₂