Patch-seq analysis of the choroid plexus epithelial cell barrier in homeostasis and in Alzheimer’s disease

The aim of this proposal is to study the blood-cerebrospinal fluid barrier (BCSFB) located at the choroid plexus (CP) level. Being the CP epithelial cells responsible for both the blood-CSF barrier formation and for the CSF production, we hypothesize that CP cells changes during aging and in Alzheimer disease (AD) may alter brain fluidics and the drainage pathways. To test this hypothesis, we are performing Patch-seq analysis, a technique that combines whole-cell patch clamp recording and scRNA-seq to draw the profile of single CP epithelial cells from wild type (WT) and AD mouse models.

Funding Agency

Cure Alzheimer’s Fund. BBEE Consortium.

Project Reference

Project Members

Main Project Outcomes

S. Queirós, “Right ventricular segmentation in multi-view cardiac MRI using a unified U-net model”, in E. Puyol Antón et al. (eds) Statistical Atlases and Computational Models of the Heart. Multi-Disease, Multi-View, and Multi-Center Right Ventricular Segmentation in Cardiac MRI Challenge. STACOM 2021. Lecture Notes in Computer Science, vol 13131, pp. 287-295, Springer, Cham, 2022.

“Best Paper Award in the M&Ms-2 Challenge”, by M&Ms2 Challenge organizers and the Medical Image Computing and Computer Assisted Intervention (MICCAI) Society.