João Luís Fernandes Machado is graduated in Health Sciences (University of Lisbon, Lisboa, Portugal) since 2019. His final project focused on the role of the S100B in EAE-Associated spinal cord pathogenesis was developed at the University of Lisbon – Instituto de Investigação do Medicamento under the supervision of Adelaide Fernandes (PhD). JLM finished his MSc studies in Neurobiology at the Faculty of Medicine of the University of Porto. His dissertation, entitled “The role of the astrocytic mGluR5 in behavior dependent on the ventral hippocampus and the prefrontal cortex” was developed at the Life and Health Sciences Research Institute (ICVS) – School of Medicine of the University of Minho under the supervision of João Filipe Oliveira (PhD). During his master, JLM generated different mouse models with astrocytic dysfunction using intracranial stereotaxic injection of viral vectors. JLM also learned and performed several behavioral tests such as the open field, elevated plus maze, tail suspension test, forced swim test, Morris water maze, two-trial place recognition task, and contextual fear conditioning. In addition, JLM performed immunohistochemical and histological analysis of mouse brain tissue as astrocytes 3D reconstruction which largely contributed to his technical expertise. JLM is currently a researcher at the ICVS – Life and Health Sciences Research Institute. JLM is currently a PhD student at the ICVS focusing his research on “in vivo” electrophysiology recording and analysis aiming to explore the role of astrocytic exocytosis.